Atomic spectrometry

The laboratory practice starts at 14.00 in room E-10. Please follow the schedule.

1.   A 15 minutes long short test is written at the beginning of the practice.

2.   The principle of atomic spectrometric techniques and the construction of atomic spectrometric instruments are demonstrated on an SP1900 (UNICAM) flame atomic absorption spectrometer in E-10 laboratory according to the schedule.

3.   The practice consists of the analysis of tap water samples using two different calibration techniques. Sampling and the dilution of standard solutions is performed in D-414 lab according to the schedule. The manual work should be performed with as little teacher’s help as possible, working in groups of 4 according to the supplementary material.

4.   The prepared standard solutions and tap water samples are measured using the FAAS and MP-AES instruments in D-423 laboratory by the groups, according to the schedule.

5.   The results should be evaluated without teacher’s help following the instructions and information in the provided supplementary material.

6.  The prepared lab reports should be submitted to D-423 laboratory, the corrected reports can be taken back 2 workdays later.

 

Possible  questions in the short test:

1.     Principle of flame spectrometric techniques (emission, absorption, fluorescence).Working principle of the given techniques and instruments.

2.     What kind of processes undergo when the solution of a metal salt is introduced to the flame?

3.     Which type of particles (atoms, ions, molecules in excited state or in ground state) contribute to the analytical signal in case of flame emission spectrometry and in case of atomic absorption spectrometry?

4.     What are the main parts of FES instrument? What are the functions of these parts?

5.     What are the main parts of FAAS instrument? What are the functions of these parts?

6.     Describe the flames used in flame spectrometry (chemical composition of the most frequently used flame gases, flame temperatures, zones in the flame)

7.     Describe the way of sample introduction of a liquid to the spectrometer

8.     Give the schematic construction and working principal of hollow cathode lamp. What is the chopper used for?

9.     What is the way of selecting the appropriate wawelenght needed for the measurement ?(Describe the resolution of the light). Give the basic principles of the light resolving unit.

10.  Describe the working principle of detectors (photomultiplier) used in atomic spectrometry.

11.  What kind of interfering effects may occur in flame spectrometry?

12.  Briefly summarize the most frequently used data evaluation techniques.

13.    What are the most important advantages and disadvantages of MP-AES compared to ICP-OES?

notes about flame spectrometry

notes about MP-AES

(notes about ICP/AES)